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Analysis Note Leupeptin gives multiple peaks on HPLC due to equilibria among three
forms in solution. Purity determined using three main peaks. Majority of contaminating
peptide is racemized leupeptin.
Biochem/physiol Actions Inhibitor of serine and cysteine proteases. Inhibits plasmin,
trypsin, papain and cathepsin B. No inhibition found with pepsin, cathepsins A and D,
thrombin, or α-chymotrypsin. Effective concentration 10-100 μM.
Enhanced Quality Profile The Enhanced Quality Profile characterizes an individual
product's quality traits in a multilevel system to assure that a given product meets the
quality requirements of our customers.
assay ≥90% (HPLC)
Stock solutions are stable up to 6 months at −20 °C.
storage temp. −20°C
Stock solutions are stable up to 6 months at −20 °C.
storage temp. −20°C
